我还是坚持我的观点。同时将current protocol上的原文贴出。



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送交者: artichaut 于 2006-2-26, 02:20:07:

回答: 可以参看这篇科普文章 由 方舟子 于 2006-2-26, 01:25:35:

我相信current protocol应该比科普作家权威的多.而且缓冻确是常规操作,我以及我周围的同学,冻细胞实际操作无不如此,而且复苏的都很好.

抱歉因为访问会有限制,没法给出current protocol的原文链接。

It is sometimes desirable to store cell lines for future study. To preserve cells, avoid senescence, reduce the risk of contamination, and minimize effects of genetic drift, cell lines may be frozen for long-term storage. Without the use of a cryoprotective agent freezing would be lethal to the cells in most cases. Generally, a cryoprotective substance such as dimethylsulfoxide (DMSO) is used in conjunction with complete medium for preserving cells at .70°C or lower. DMSO acts to reduce the freezing point and allows a slower cooling rate. Gradual freezing reduces the risk of ice crystal formation and cell damage.
(Unit 1.1.3, Current Protocols in Cell Biology,1998)



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