Many thanks for your suggestion. I failed b4 the sepharose. Nothing came off.

送交者: Wood 于 2006-07-30, 17:59:16:

回答: Some suggestion 由 lijun 于 2006-07-30, 17:46:21:

Normally, I get max 5mg protein from 60 L culture (for growing crystals).

By the way, how much does a Sephadex 200 > 2m cost?

Before Sepharose? - lijun (324 bytes) 2006-07-30, 19:48:44 (96036)
Sounds like it's a recombinant protein - lijun (346 bytes) 2006-07-30, 19:24:26 (96035)
- a 2 m column only $300, £160? A 30 cm cost me £110. (无内容) - Wood (0 bytes) 2006-07-30, 21:08:23 (96059)
- A native bacterial protein. DE-52, P.Seph. SEC then denature, refold, - Wood (13 bytes) 2006-07-30, 20:46:14 (96054)
  - Failed at DE-52? - lijun (262 bytes) 2006-07-31, 08:39:01 (96162)
  - You may replace the P-Seph with a Butyl-seph. column. (无内容) - lijun (0 bytes) 2006-07-31, 08:31:31 (96161)