Failed at DE-52?
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送交者: lijun 于 2006-07-31, 08:39:01:
回答: A native bacterial protein. DE-52, P.Seph. SEC then denature, refold, 由 Wood 于 2006-07-30, 20:46:14:
DE-52 is difficult to control if you didn't adjust pH of the resin. You need to swell the resin in buffer and adjust pH to your desired value before packing it. That's why I suggested you to use Q-sepharose instead, which is easier to use and more reproduciable.
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