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　　悲剧啊，丘小庆，我为您汗颜！ 

　　作者：无名氏

　　拜读大作“An engineered multidomain bactericidal peptide as a 
model for targeted antibiotics against specific bacteria”方法部分顿生
疑云：

　　１. "Harvested plasmid was transfected into TG1 E. coli cells to 
produce pheromonicin. TG1 cells harboring pheromonicin plasmids were 
grown in FB medium containing 50 g/ml ampicillin (to select for the 
plasmid) ..."

　　兄弟啊，这么重要的文章您竟然没有测序去确认YSTCDFIM在里面啊？ 

　　２. "...were resuspended in 60_80 ml borate buffer (50 mM borate 
buffer, pH 9.0, with 2 mM EDTA and 2 mM dithiothreitol) containing 0.5 
mM phenylmethylsulfonyl fluoride.　

　　您老兄知不知道colicin Ia 的理论等电点是多少？９.１５（Theoretical 
pI: 9.15).　加了后面那八个氨基酸后也变不到那里去。 你老人家用50 mM 
borate buffer, pH 9.0去提，您提什么？您去翻翻那生化入门再当教授吧！

　　３.　The cells were sonicated and debris removed by centrifugation 
for 90 min at 75,000g. 

　　您这一上来便是超离，闻所未闻！ 

　　４.　Nucleic acids were removed by addition of 1/5 volume 
streptomycin sulfate. 

　　兄弟啊，有更简单的办法去ＤＮＡ／ＲＮＡ，您是故意加进链霉素留下伏笔
啊，日后若被查出好说是实验错误而不是造假！这是通篇文章的最高明之处。佩
服佩服。

　　５.　Dialyzed extracts were applied to a CM-Sepharose column (2.5  
12 cm; Pharmacia Biotech).　Proteins were recovered by stepwise 
elution with 0.1, 0.2 and 0.3 M NaCl in borate buffer and collected in 
0.5-ml fractions16, 17. The total protein concentration of eluate was 
about 5 mg/ml. 　

　　您这一步离子交换就得到了纯蛋白？神人也！

　　６.　As determined from 15% SDS-polyacrylamide gel assays, 
pheromonicin eluted by 0.2 M NaCl comprised about 90% of total eluted 
protein (Fig. 1c).　

　　您那７０　kDa　的蛋白用１５％　ＳＤＳ－ＰＡＧＥ？可悲阿！

　　川大啊川大，贵学府怎会有如此的教授？此文若不是造假才怪呢！谁能重复
出来？ 

　　没人提及另一通讯作者　ＧＥＯＲＧＥ　Ｙ　ＷＵ　（Division of 
Gastroenterology-Hepatology, University of Connecticut Health Center, 
Farmington, Connecticut 06030-1845, USA），他也脱不了干系。

(XYS20060125)

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