送交者: trus 于 2006-5-03, 09:33:07:
实验中使用的是35aa多肽，请问这么短的多肽不和大分子载体耦联成功产生抗体的几率有多大？
ynthesis of peptides and immunization with the peptides.
Peptides were synthesized by FMOC (fluorenyl methoxycarbonyl)-t-butyl-based solid-phase peptides chemistry on an AB433A peptide synthesizer (Perkin Elmer, Norwalk, Connecticut) as described45. The crude peptides were purified by reverse-phase high-performance liquid chromatography44.
Peptides (100 g antigen per injection) were emulsified 1:1 (volume/volume) with complete Freund's adjuvant for the first immunization, followed by a boost in incomplete Freund's adjuvant at 2 weeks and weekly thereafter, as described46, then challenged with 1 105−1 107 live tumor cells after the fourth immunization. Purified immunoglobulins were prepared from the pooled sera derived from the mice at day 7 after the fourth immunization without injection of tumor cells.