送交者: whatsup 于 2006-4-27, 23:48:01:
Author Name task started on Fri Apr 28, 2006 at 12:39 AM
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wei, yuquan
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WEI YUQUAN (90 references)
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Bibliographic Information
Antitumor effects of interferon- -inducible protein 10 combined with gemcitabine. Mei, Kai; Tian, Ling; Wei, Yuquan; Li, Jiong; Wen, Yanjun; Kan, Bing; Deng, Hongxin. West China Hospital, Sichuan University, Chengdu, Sichuan Province, Peop. Rep. China. Aizheng (2005), 24(4), 397-402. Publisher: Sun Yat-sen Daxue, Aizheng Zhongxin, CODEN: AIZHE4 ISSN: 1000-467X. Journal written in Chinese. AN 2006:221455 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The antitumor effects of IP-10 (interferon -inducible protein-10, an inhibitor of tumor angiogenesis) combined with gemcitabine in tumor-bearing mice were investigated to explore their synergistic effects. Hepatocarcinoma H22 model was established in BALB/c mice; Lewis lung cancer LL/2 model was established in C57BL/6 mice. The mice were divided into 4 groups: treated with IP-10, gemcitabine, IP-10 plus gemcitabine, and normal saline (the control). After treatment of IP-10 plasmid, IP-10 protein reached the peak level of (16.83.6) ng/mL at the 14th day, and remained a high level of (14.02.1) ng/mL at the 35th day. Compared with the controls, tumor vol. of mice in the combination therapy group had significant regression, or disappeared. Nine weeks after inoculation of tumor cells, the survival rate of mice was significantly higher in combination group than in IP-10, gemcitabine and the control groups (90% vs. 55%, 0% and 0%). No obvious side effects were obsd. MVD of tumor tissues was significantly lower in combination group than in IP-10, gemcitabine and the control groups (15.82.4 vs. 45.62.0, 50.23.5 and 51.33.0). At the 35th day after inoculation of tumor cells, the apoptotic index was significantly higher in combination group than in IP-10, gemcitabine and the control groups (85.510.2 vs. 21.45.5, 8.42.0 and 4.20.7). Therapy of IP-10 combined with gemcitabine had significantly synergistic antitumor effects compared with IP-10 or gemcitabine alone.
Bibliographic Information
Antitumor effects of recombinant quail vascular endothelial growth factor receptor 2, as a vaccine, combined with cisplatin on LL/2 tumor model in mice. Hou, Jianmei; Liu, Jiyan; Yang, Li; Zhao, Xia; Tian, Ling; Lei, Song; Mao, Yongqiu; Wen, Yanjun; Wei, Yuquan. West China Hospital, Sichuan University, Chengdu, Sichuan Province, Peop. Rep. China. Aizheng (2005), 24(4), 391-396. Publisher: Sun Yat-sen Daxue, Aizheng Zhongxin, CODEN: AIZHE4 ISSN: 1000-467X. Journal written in Chinese. AN 2006:221427 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The antitumor effects of recombinant quail vascular endothelial growth factor receptor 2 (qVEGFR2) combined with cisplatin on tumor-bearing mice were investigated to explore the new strategy of bio-chemotherapy of tumor. Lewis lung carcinoma model (LL/2) was established in C57BL/6 mice. Seven days after inoculation of tumor cells, the mice were randomized into combination group, qVEGFR2 group, chemotherapy group and normal saline (NS) group. Tumor vol. of mice was obviously smaller in combination group than in NS group. The complete regression of tumor growth was obsd. in 3 of the 10 mice in combination group. Seventy days after inoculation of tumor cells, the survival rate of mice was significantly higher in combination group than in qVEGFR2, cisplatin and NS groups (90% vs. 60%, 0% and 0%). Anti-VEGFR-2 antibody-producing B cells (APBCs) counts were (156.819.3)/106 spleen cells in combination group, and (143.618.6)/106 spleen cells in qVEGFR group. MVD (microvessel d.) was significantly lower in combination group than in cisplatin and NS groups (11.41.3 vs. 33.44.5 and 45.54.5), while it in qVEGFR2 group was 16.41.6. Apoptosis was significantly higher in combination group than in qVEGFR2, cisplatin and NS groups (36.2% vs. 15.4%, 17.6% and 4.1%). The combination therapy of qVEGFR2 and cisplatin had synergistic antitumor effects.
Bibliographic Information
Proteomics profile changes in cisplatin-treated human ovarian cancer cell strain. Li, Zhengyu; Zhao, Xia; Yang, Jinliang; Wei, Yuquan. Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, Peop. Rep. China. Science in China, Series C: Life Sciences (2005), 48(6), 648-657. Publisher: Science in China Press, CODEN: SCCLFO ISSN: 1006-9305. Journal written in English. AN 2006:166217 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
To compare the alterations in proteomes between cisplatin-treated and -untreated human ovarian cancer SKOV3 cells, and to explore the feasibility of proteomics in research about antitumor mechanisms of agents, SKOV3 cells were exposed to cisplatin (6 g/mL) for 6 h. Then, the cells were collected and solubilized and global proteins were extd. by lysis buffer; two-dimensional electrophoresis was conducted with the IPG readystrips as carriers; the gels were stained with Coomassie blue and alterations between gels were compared by PDQuest. Eventually, 11 spots with significant differences were selected and excised and the proteins were identified by PMF and MS/MS anal. The results revealed that exposure to cisplatin could notably increase expressions of some proteins, such as tropomyosin family, actin family, triosephosphate isomerase family, and HSP60, etc.; while expressions of some other proteins decreased, such as enolase family, etc. Those proteins were involved in cellular energy metab., transformation, apoptosis and morphol. maintenance, which suggested that alterations of those physiol. processes might be involved in anti-tumor mechanism of cisplatin.
Bibliographic Information
Prokaryotic expression, purification and identification of recombinant Xenopus laevis and mouse vascular endothelial growth factors. Niu, Ting; Liu, Ting; Jia, Yongqian; Yang, Li; Tian, Ling; Liu, Jiyan; Hu, Bing; Wu, Yang; Wei, Yuquan. West China Hospital, Sichuan University, Chengdu, Peop. Rep. China. Sichuan Daxue Xuebao, Yixueban (2005), 36(3), 301-304. Publisher: Sichuan Daxue Xuebao, Yixueban Bianjibu, CODEN: SDXYAY ISSN: 1672-173X. Journal written in Chinese. AN 2006:41346 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The prokaryotic expression vectors were constructed by restriction endonuclease digestion and ligation, then the recombinant vector pEX-xVEGF and the control vector pET-mVEGF were identified by restriction enzymic digestion and DNA sequencing. The confirmed vectors were transformed into Escherichia coli. BL21 (DE3) and recombinant protein expression was induced by Isopropyl--D-thiogalactoside. The recombinant xVEGF and mVEGF were obtained and purified by Ni-NTA affinity chromatog. under denature conditions and enterokinase specific digestion, resp. Finally, the purified proteins' mol. wts. and specificity were detected by SDS-PAGE and western blot anal. Two new recombinant expression vectors, pEX-xVEGF and pET-mVEGF, were constructed successfully. The xVEGF and mVEGF fusion proteins were expressed in E. coli BL21 (DE3) stably, and the mol. wts. of the purified xVEGF and mVEGF were identical to the expected values. The purity of final products reached a level higher than 95%. In addn., these two purified proteins could react with a specific antibody against mouse VEGF as expected. Recombinant Xenopus laevis VEGF and its control mouse VEGF protein may provide tools for further study of anti-tumor active immunity with this xenogeneic protein vaccine in mouse tumor models.
Bibliographic Information
Cloning FGFR-1 cDNA of Drosophila and construction its expression plasmids. Mao, Yongqiu; He, Qiuming; Luo, Feng; Jiang, Yu; Wei, Yuquan. West China Hospital, Sichuan University, Chengdu, Peop. Rep. China. Sichuan Daxue Xuebao, Yixueban (2005), 36(3), 432-433, 440. Publisher: Sichuan Daxue Xuebao, Yixueban Bianjibu, CODEN: SDXYAY ISSN: 1672-173X. Journal written in Chinese. AN 2006:41244 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Aim: Fibroblast growth factor receptor-1 (FGFR-1) cDNA was cloned, and its eucaryotic expression plasmid pdFR1 was constructed. Methods: Drosophila FGFR-1 gene was amplied by PCR, and was cloned into plasmid pT-Adv, and then it was transfered into Escherichia coli XL1-Blue. The recombinant plasmid pT-Adv/FGFR-1 was digested; the target gene was cloned into eucaryotic expression plasmid pcDNA3.1(+), named pdFR1. The products was identified by DNA sequencing. Results: The DNA sequencing of pT-Adv/FGFR-1 was the same with the reported sequence. The fragments digested with endonuclease were as large as the predicted results. Conclusion: The Drosophila FGFR-1 full length gene was cloned and its eucaryotic expression plasmid pdFR1 was constructed.
Bibliographic Information
Serum proteomics study of the squamous cell carcinoma antigen 1 in tongue cancer. Huang, Xin; Wei, Yuquan; Li, Longjiang; Wen, Yuming; Yang, Jingliang; Liu, Bin; Song, Xin; Zhao, Jian. State Key Laboratory of Biotherapy, Sichuan University, Chendu City, Peop. Rep. China. Oral Oncology (2006), 42(1), 26-31. Publisher: Elsevier Ltd., CODEN: EJCCER ISSN: 1368-8375. Journal written in English. AN 2005:1348475 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The identification of serum biomarkers as a means of the early diagnosis and finding possible therapeutic targets in cancers is of increasing interest. In the present study, cells of human tongue cancer cell line Tca8113 were s.c. inoculated into nude mice, while control nude mice were injected with phosphate-buffered saline. Two weeks after injection, serum from mice was collected for proteomic anal. using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). Comparing the serum 2-DE maps from the tumor-bearing mice with those produced from control mice, we found that squamous cell carcinoma antigen 1 was over-expressed only in tumor-bearing mice. Squamous cell carcinoma antigen 1 was also up-regulated in clin. tongue cancer patients by RT-PCR and Western-blotting. These results indicate that squamous cell carcinoma antigen 1 may be of great potential as the biomarker of tongue cancer and as the potential therapeutic target for gene therapy.
Bibliographic Information
Fusion expression, purification and bioassay of interferon- (IFN- ) inducible protein-10 and thioredoxin gene in Escherichia (E.) coli. Li, Gang; Tian, Ling; Wei, Yuquan; Wen, Yanjun; Xiao, Fei; Yao, Bing; Zhang, Ling; Zhang, Ru; Mei, Kai. West China Hospital, Sichuan University, Chengdu, Peop. Rep. China. Shengwu Yixue Gongchengxue Zazhi (2005), 22(3), 535-539. Publisher: Shengwu Yixue Gongchengxue Zazhi, CODEN: SYGZF2 ISSN: 1001-5515. Journal written in Chinese. AN 2005:1342726 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Interferon -inducible protein 10 (IP-10), a member of CXC chemokine family, was secreted by interferon gamma-stimulated monocytes, endothelial cells and keratinocytes. IP-10 played an important role in recruiting activated T cells into sites of tissue inflammation. In this expt., PCR products of IP-10 gene were cloned into prokaryotic expression vector pET 32(a) to generate recombinant pET-IP10 with N-terminal S-Tag, and then the target protein was expressed successfully in Escherichia (E.) coli BL21 (DE3). The total expressed products amounted to 25.3% of the total bacterial proteins. The expressed protein of pET-IP10 mainly formed inclusion body in E. coli. Sol. recombinant protein accounted for 20% of the IP-10 fusion protein. The sol. recombinant proteins were purified using S-Tag affinity chromatog. effectively with purity of over 90%. The chemotaxis activity of purified IP-10 could specifically exhibit the directional migration of stimulated T cells at concn. of 100 ng/mL. The strategy used in this expt. was effective for recombinant IP-10 prodn. with biol. activity.
Bibliographic Information
Expression of xenogenic homologous epidermal growth factor receptor ectodomain in Pichia pastoris. Fang, Fang; Li, Jiong; Wen, Yanjun; Tian, Ling; Wei, Yuquan. West China Hospital, Sichuan University, Chengdu, Peop. Rep. China. Sichuan Daxue Xuebao, Yixueban (2005), 36(2), 153-156. Publisher: Sichuan Daxue Xuebao, Yixueban Bianjibu, CODEN: SDXYAY ISSN: 1672-173X. Journal written in Chinese. AN 2005:1205321 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A secreted expression system for expressing xenogenic homologous epidermal growth factor receptor (EGFR) ectodomain in Pichia pastoris (P. pastoris) was developed to provide a basis for subsequent antitumor immunol. researches on EGFR subunit protein vaccine. In order to construct the secreted expression vector pPICZaA-mer and pPICZaA-her, the cDNA of xenogenic (human) EGFR ectodomain (her) and the corresponding control mouse EGFR ectodomain (mer) were recombined with the empty plasmid pPICZaA, resp. Then the recombinant plasmids were linearized by Sac I and were transformed into P. pastoris strain GS115 by electroporation. The pos. transformants were selected on YPDS plate including Zeocin and were induced to express by methanol. Consequently, the proteins in the culture supernatant were assayed with SDS-PAGE and Western-blot. The high-level secreted expression recombinant strain was chosen to produce protein vaccine vastly. The secreted expression of mouse and human EGFR ectodomain was found in P. pastoris. The relative mol. mass of the two aimed proteins was about 95103. The Western-blot anal. demonstrated that the expression proteins had much good antigenicity and specificity. The recombinant plasmids pPICZaA-mer and pPICZaA-her could be induced to express the EGFR aimed proteins by methanol in methanol-trophic yeast expression system.
Bibliographic Information
A cell filter. Lu, Zuhong; Wei, Yuquan; Xiao, Pengfeng; Zhang, Shengyou. (Southeast University, Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu (2005), 9 pp. CODEN: CNXXEV CN 1560223 A 20050105 Patent written in Chinese. Application: CN 1001-4077 20040217. Priority: . AN 2005:1073392 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Patent Family Information
Patent No. Kind Date Application No. Date
CN 1560223 A 20050105 CN 2004-10014077 20040217
Priority Application
CN 2004-10014077 20040217
Abstract
The invention relates to a microfluid component, i.e. a cell filter, for the filtration and sepn. of cells. The cell filter comprises a sealed fluid pathway as cavity 1, fluid inlet 2 and fluid outlet 3 disposed resp. at two sides of the cavity, a cell granulation barrier 4 disposed between the fluid inlet and outlet, and a fluid pond 5 disposed next to the barrier. The cell granulation barrier can block larger cells in fluid, and accumulate these cells into the fluid pond where these cells are captured by the corresponding protein. By applying phys. or chem. action, the larger cells are crushed or deactivated to realize cell filtration. The filter has good applications in removing cancer cells in blood, reducing the risk of carcinomatous metastasis after operation, inhibiting the cancer diffusion and recurrence, and improving the curative effects on the treatment of cancer.
Bibliographic Information
Syntheses of carbamate derivatives of quercetin by reaction with amino acid ester isocyanates. Wu, Xianxue; Cheng, Li; Xiang, Dong; Wei, Yuquan. College of Chemistry, Sichuan University, Chengdu, Peop. Rep. China. Letters in Organic Chemistry (2005), 2(6), 535-538. Publisher: Bentham Science Publishers Ltd., CODEN: LOCEC7 ISSN: 1570-1786. Journal written in English. CAN 144:311813 AN 2005:943564 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Synthesis of amino acid carbamate derivs. of quercetin, e.g. I, from partly protected quercetin and amino acid esters utilizing triphosgene as an auxiliary reagent was studied. A series of quercetin derivs. were obtained regioselectively by reaction with amino acid ester isocyanates under mild conditions and in high yields.
Bibliographic Information
T cell homeostatic proliferation associated with autoimmunity and antitumor immunity. Hu, Bing; Wei, Yuquan. West China Hospital, Sichuan University, Chengdu, Sichuan Province, Peop. Rep. China. Mianyixue Zazhi (2004), 20(3), 239-241. Publisher: Mianyixue Zazhi Bianjibu, CODEN: MIZAED ISSN: 1000-8861. Journal; General Review written in Chinese. CAN 144:49683 AN 2005:920980 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A review. Through pos. and neg. selection, thymocytes with high avidity for self peptide-major histocompatibility complex (MHC) are eliminated, but retaining low yet significant reactivity for self-peptides. After maturing and leaving the thymus, these cells continue to depend on the interaction of T cell receptor (TCR) with self peptide-MHC complex for survival signals. Maturing T cells are regulated by homeostatic proliferation for the total size of the T cell pool at a const. level, esp. at the final stage of an immune response and lymphopenia status. Homeostatic proliferation requires interaction between TCR and self peptide-MHC complex with lower avidity. Homeostatically proliferated T cells show both effector and memory characteristics against autoantigens, and are sufficient to induce autoimmune disease. On the other hand, these characteristics also could be explored for the immunotherapy targeted against nonmutated tumor-assocd. self-antigens.
Bibliographic Information
Potential anticancer activity of tanshinone IIA against human breast cancer. Wang, Xiujie; Wei, Yuquan; Yuan, Shulan; Liu, Guanjian; Lu, Yanrong; Zhang, Jie; Wang, Wendong. Key Laboratory of Biotherapy of Human Diseases, West China Hospital, Ministry of Education, Sichuan University, Chengdu, Peop. Rep. China. International Journal of Cancer (2005), 116(5), 799-807. Publisher: Wiley-Liss, Inc., CODEN: IJCNAW ISSN: 0020-7136. Journal written in English. CAN 143:278640 AN 2005:842977 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Tanshinone IIA is a deriv. of phenanthrene-quinone isolated from Danshen, a widely used Chinese herbal medicine. It has antioxidant properties and cytotoxic activity against multiple human cancer cell lines, inducing apoptosis and differentiation of some human cancer cell lines. Our purpose was to confirm its anticancer activity on human breast cancer in vitro and in vivo and to elucidate the mechanism of its activity. Human breast cancer cells were tested in vitro for cytotoxicity, colony formation inhibition, BrdU incorporation and gene expression profiling after treatment with tanshinone IIA. Seven nude mice bearing human breast infiltrating duct carcinoma orthotopically were tested for anticancer activity and expression of caspase-3 in vivo by s.c. injection of tanshinone IIA at a dose of 30 mg/kg 3 times/wk for 10 wk. Tanshinone IIA demonstrated a dose- and time-dependent inhibitory effect on cell growth (IC50 = 0.25 g/mL), and it significantly inhibited colony formation and BrdU incorporation of human breast cancer cells. Oligonucleotide microarray anal. identified 41 upregulated (1.22%) and 24 downregulated (0.71%) genes after tanshinone IIA treatment. Upregulated genes were involved predominantly in cycle regulation, cell proliferation, apoptosis, signal transduction and transcriptional regulation; and downregulated genes were assocd. mainly with apoptosis and extracellular matrix/adhesion mols. A 44.91% tumor mass vol. redn. and significant increase of caspase-3 protein expression were obsd. in vivo. Our findings suggest that tanshinone IIA might have potential anticancer activity on both ER-pos. and -neg. breast cancers, which could be attributed in part to its inhibition of proliferation and apoptosis induction of cancer cells through upregulation and downregulation of multiple genes involved in cell cycle regulation, cell proliferation, apoptosis, signal transduction, transcriptional regulation, angiogenesis, invasive potential and metastatic potential of cancer cells.
ADPRTL1 might be the main target at which tanshinone IIA acted.
Bibliographic Information
Cancer prevention and anticancer activity of proanthocyanidins. Wang, Xiujie; Yuan, Shulan; Wei, Yuquan. West China Hospital, Sichuan University, Chengdu, Sichuan Province, Peop. Rep. China. Zhongcaoyao (2004), 35(7), 830-833. Publisher: Zhongcaoyao Zazhi Bianjibu, CODEN: CTYAD8 ISSN: 0253-2670. Journal; General Review written in Chinese. CAN 143:415401 AN 2005:841254 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A review on proanthocyanidins (PA) including 1) its sources and varieties, 2) its anticancer activity, 3) inhibition of PA on human tumor cell proliferation, 4) the mechanism of PA anticancer effects, 5) problems and prospects.
Bibliographic Information
Concanavalin A induced murine model for hepatic fibrosis. Li, Hongli; Tian, Ling; Wei, Yuquan; Zhao, Xia. West China Hospital, Sichuan University, Chengdu, Sichuan Province, Peop. Rep. China. Mianyixue Zazhi (2004), 20(5), 390-392, 396. Publisher: Mianyixue Zazhi Bianjibu, CODEN: MIZAED ISSN: 1000-8861. Journal written in Chinese. CAN 143:344299 AN 2005:528789 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A murine model for hepatic fibrosis with Con A induction was established. Twenty Balb/c mice were randomly divided into 2 groups. In the exptl. group (E), the mice were injected with a soln. contg. Con A at a dose of 12.5 mg/kg once a week for 6 wk. In the control group (N), the mice were injected with 250 L pyrogen-free PBS instead of Con A. Serum was extd. from individual mouse through the tail vein at 24 h after the injection. Hepatocellular injuries were evaluated by measuring serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity. The mice were sacrificed at 1 wk after the sixth/final injection of Con A. The morphol. changes and collagen deposition of the liver tissue were obsd. by microscope after hematoxylin & eosin staining and Masson Trichrome staining. In contrast with group N, the levels of ALT and AST in group E were significantly increased, the vols. of liver enlarged remarkably, external surface of the liver was rough and studded with regenerated nodules. Destruction and necrosis of hepatocytes, and invasion of lymphocytes were found by microscopy. In addn., Masson Trichrome staining showed that the collagen deposition in liver parenchyma was obviously increased. A murine model for hepatic fibrosis can be established by repeatedly administration of Con A.
Bibliographic Information
Structure, function and signal transduction of vascular endothelial growth factor receptor-2. Liu, Jiyan; Wei, Yuquan. West China Hospital, Sichuan University, Chengdu, Sichuan Province, Peop. Rep. China. Zhongguo Bingli Shengli Zazhi (2004), 20(7), 1309-1313. Publisher: Jinan Daxue, CODEN: ZBSZEB ISSN: 1000-4718. Journal; General Review written in Chinese. CAN 143:279488 AN 2005:435823 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A review on the structure, function and signal transduction of vascular endothelial growth factor receptor-2 (VEGFR-2).
Bibliographic Information
Encapsulated substance that can release continuously 2 kinds of vasostatins and its preparation. Wei, Yuquan; Xiao, Fei; Zhao, Xia; Yang, Li. (Sichuan University, Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu (2003), 14 pp. CODEN: CNXXEV CN 1431017 A 20030723 Patent written in Chinese. Application: CN 2002-133546 20020730. Priority: . CAN 142:469271 AN 2005:414467 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Patent Family Information
Patent No. Kind Date Application No. Date
CN 1431017 A 20030723 CN 2002-133546 20020730
Priority Application
CN 2002-133546 20020730
Abstract
The encapsulated substance with size of 300-500 m is the alginate-encapsulated human vasostatin gene/human sFLT-1 gene- carrying eukaryotic expression vector pcDNA3.1(+) or pSecTag2B- transfected 293 cell or A549 cell, and the content of the cell in the encapsulated substance is (0.2-20) x 106 mL-1. The vasostatin is the amino acid fragment at 1-180 position of N-terminal of human calreticulin, and sFLT-1 is 1st-6th Ig-like domains of the extracellular segment of human sol. vascular endothelial growth factor receptor-1. The human vasostatin gene/human sFLT-1 gene-carrying eukaryotic expression vector-transfected cell is constructed by PCR amplifying the human vasostatin cDNA fragment from human skeletal muscle cDNA library, inserting into the vector pUC18 to obtain recombinant plasmid pUC18-vaso; cloning the sFLT-1 cDNA fragment from human umbilical cord venous endothelial cell cDNA library, inserting into the vector PCR2.1-TOPO Ta to obtain recombinant plasmid p2.1-sflt; subcloning the vasostatin gene fragment into vector pSecTag2B to obtain recombinant plasmid p2B-vaso, subcloning the sFLT-1 gene fragment into plasmid pcDNA3.1(+) to obtain recombinant plasmid pc3.1-sflt; co-transfecting into 293 cell or A549 cell, and identifying. The encapsulated substance may be used for inhibiting the generation, development, and metastasis of tumor.
Bibliographic Information
Active Antitumor Immunity Elicited by Vaccine Based on Recombinant Form of Epidermal Growth Factor Receptor. Hu, Bing; Wei, Yuquan; Tian, Ling; Zhao, Xia; Lu, You; Wu, Yang; Yao, Bing; Liu, Jiyan; Niu, Ting; Wen, Yanjun; He, Qiuming; Su, Jingmei; Huang, Meijuan; Lou, Yanyan; Luo, Yan; Bing, Kan. State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Sichuan, Peop. Rep. China. Journal of Immunotherapy (2005), 28(3), 236-244. Publisher: Lippincott Williams & Wilkins, CODEN: JOIMF8 ISSN: 1524-9557. Journal written in English. CAN 143:113688 AN 2005:336698 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Active immunotherapy targeting epidermal growth factor receptor (EGFR) should be another attractive approach to the treatment of EGFR-pos. tumors. To test this concept, the authors evaluated the potential immune responses and antitumor activities elicited by dendritic cells pulsed with recombinant ectodomain of mouse EGFR (DC-edMER). Spleen cells isolated from DC-edMER-vaccinated mice showed a high quantity of EGFR-specific antibody-producing cells. EGFR-reactive antibody in sera isolated from vaccinated mice was identified and shown to be effective against tumors in vitro and in vivo by adoptive transfer. DC-edMER vaccine also elicited cytotoxic T-lymphocyte responses that could mediate antitumor effects in vitro and adoptive transfer in vivo. In addn., EGFR-specific cytokines responses were elicited by DC-edMER vaccine. Immunization with DC-edMER resulted in tumor regression and prolonged survival in mice challenged with Lewis lung carcinomas and mammary cancer models. Depletion of CD4+ T lymphocytes could completely abrogate the antitumor activity and EGFR-specific antibody responses, whereas the depletion of CD8+ T lymphocytes showed partial abrogation of the antitumor activity but antibody was still detected. Furthermore, tumor-induced angiogenesis was suppressed in DC-edMER-vaccinated mice or mice treated with antibody adoptive transfer. Thus, antitumor immunity could be induced by DC-edMER, which may involve both humoral and cellular immunity, and may provide insight into the treatment of EGFR-pos. tumors through the induction of active immunity against EGFR.
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Screening and identification of stable transfectants of mouse soluble B lymphocyte stimulator. Fu, Chunhua; Tian, Ling; Wei, Yuquan; Kan, Bing; Li, Jiong; Wen, Yanjun. West China Hospital, Sichuan University, Chengdu, Peop. Rep. China. Shengwu Yixue Gongchengxue Zazhi (2004), 21(6), 897-900. Publisher: Shengwu Yixue Gongchengxue Zazhi, CODEN: SYGZF2 ISSN: 1001-5515. Journal written in Chinese. CAN 143:171014 AN 2005:306564 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Mouse colon cancer cells CT26 were transfected with constructed plasmid expressing mouse sol. B lymphocyte stimulator (msBlyS). Single cell clones were selected with 100 g/mL Zeosin and subcloned by serial limiting diln. Eight resistant transfectants were isolated and expanded, and five of them displayed the desirable msBlyS cDNA band amplified by semi-quant. RT-PCR assay. Western blot anal. showed that only msBlyS mols. of the expected size were detected in the cell lysates from transfectants. The supernatant of transfectants could costimulate B cell proliferation in std. costimulation assay. Thus the authors have successfully screened the stable transfectants expressing high levels of msBlyS in CT26 cells, which could be used as cancer vaccines for further anti-tumor immunotherapy.
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Immunotherapy of tumor by targeting angiogenesis. Hou, Jianmei; Tian, Ling; Wei, Yuquan. Key Laboratory of Biotherapy of Human Diseases of Ministry of Education, West China Hospital of Sichuan university, Chengdu, Peop. Rep. China. Science in China, Series C: Life Sciences (2004), 47(6), 545-552. Publisher: Science in China Press, CODEN: SCCLFO ISSN: 1006-9305. Journal; General Review written in English. CAN 142:334515 AN 2005:136917 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A review. Tumor growth and metastasis are angiogenesis-dependent. Anti-angiogenic therapy represents a new strategy for the development of anti-cancer therapies. In recent years, there has been made great progress in anti-angiogenic therapy. As far as the passive immunotherapy is concerned, a recombinant humanized antibody to vascular endothelial growth factor (VEGF), Avastin, has been approved by FDA as the first angiogenesis inhibitor to treat colorectal cancer. For active specific immunotherapy, various strategies for cancer vaccines, including whole endothelial cell vaccines, dendritic cell vaccines, DNA vaccines, and peptides or protein vaccines, have been developed to break immune tolerance against important mols. assocd. with tumor angiogenesis and induce angiogenesis-specific immune responses. The authors review the angiogenesis-targeted immunotherapy of tumors from the above two aspects.
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Construction of recombinant adenovirus for the angiogenic inhibitor, Vasostatin, and its expression in vitro. Ding, Zhenyu; Yang, Li; Tian, Ling; Liu, Fen; Xiao, Fei; Wei, Yuquan. West China Hospital, Sichuan University, Chengdu, Peop. Rep. China. Sichuan Daxue Xuebao, Yixueban (2004), 35(4), 460-462, 469. Publisher: Sichuan Daxue Xuebao, Yixueban Bianjibu, CODEN: SDXYAY ISSN: 1672-173X. Journal written in Chinese. CAN 143:299825 AN 2005:80289 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Objective: to construct a recombinant replication-deficient adenovirus for the angiogenic inhibitor, vasostatin and to assay its expression in vitro. Methods: the cDNA for vasostatin was got by PCR amplification, then it was cloned into T vector and confirmed by enzymic anal. and direct sequencing. Subsequently the cDNA was subcloned into the shuttle plasmid, and co-transformed into 293 cells with backbone plasmid. The resulting recombinant andenovirus was confirmed by PCR and western blot. The activity was assayed by inhibition of HUVEC growth. Results: the PCR product was about 590 bp in length, and the sequencing result was identical to that reported. The construction of recombinant replication-deficient adenovirus was confirmed by PCR. Western blot anal. showed the expression of vasostatin in vitro. The supernatant from transduced HeLa cells inhibited the growth of HUVEC specifically. Conclusion: the recombinant adenovirus for vasostatin efficiently mediated the expression of the protein in vitro.
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Inhibition of tumor growth and metastasis via local administration of recombinant human endostatin adenovirus. Wu, Yang; Yang, Li; Zhao, Xia; Su, Jingmei; Hu, Bing; Liu, Jiyan; Niu, Ting; Luo, Yan; Li, Qiu; Wei, Yuquan. West China Hospital, Sichuan University, Chengdu, Sichuan Province, Peop. Rep. China. Zhonghua Yixue Yichuanxue Zazhi (2004), 21(6), 557-561. Publisher: Huaxi Yike Daxue, CODEN: ZYXZER ISSN: 1003-9406. Journal written in Chinese. CAN 142:309365 AN 2005:61334 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A topical antiangiogenic gene therapy with recombinant human endostatin adenovirus (Ad-hEndo) was designed and its effects on the inhibition of angiogenesis in vitro, and tumor growth and metastasis in vivo were assessed. Malignant cells (A549) were infected with Ad-hEndo. The expression of recombinant protein and the inhibition of cultured human umbilical vein endothelial cells were investigated. Immunodeficient A549 nude mice were treated with intratumoral injection of Ad-hEndo, the empty vector Ad-control or saline (NS). The dose-response, side effects, and serum concn. of endostatin were obsd. Recombinant endostatin protein was detected in the infected tumor cells with different MOI Ad-hEndo and its inhibitory effect on endothelial cells growth was shown. In animal study, the vol. of tumor and the no. of pulmonary metastatic lesions in the Ad-hEndo treatment group were significantly smaller than those in the control groups (P0.05). One hour after the ECC, the level of CD11b+/CD18+ in group B was lower than that in group A, that in group C was lower than that in group B, and that in group D was higher than that in group C, but no significant difference between groups was obsd. (P >0.05). Although amrinone and aprotinin had anti-inflammatory activity, the pump prime to which was added aprotinin alone or aprotinin combined with amrinone might fail in preventing the expression of leukocyte adhesion mol. CD11b/CD18 completely in patients with prosthetic valve replacement during ECC perioperative period.
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Study on the Expression of Adhesion Molecule Related Proteins in Patients with Coronary Heart Disease. Chen, Xiaoping; Zhang, Dingbao; Yang, Qing; Huang, Dejia; Zhang, Li; Gao, Mei; Lei, Song; Huang, Minghui; Liu, Xiaojing; Wei, Yuquan. Department of Cardiology, West China Hospital, Sichuan University, Chengdu, Peop. Rep. China. Huaxi Yike Daxue Xuebao (2002), 33(2), 276-277, 290. Publisher: Huaxi Yike Daxue, CODEN: HYDXET ISSN: 0257-7712. Journal written in Chinese. CAN 138:219380 AN 2002:884322 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The expression level of adhesion mol. related proteins CD11a and CD11b on neutrophils, monocytes, and lymphocytes in patients with coronary heart disease and its relation to the degree of stenosis of coronary arteries and severity of coronary heart disease were studied. The subjects were divided into three groups, stable angina, unstable angina, and healthy people as control. The amt. of cell adhesion mol. related proteins CD11a and CD11b on neutrophilic granulocytes, monocytes, and lymphocytes of peripheral blood was measured in three groups by using fluorescent immunoassay method on flow cytometer. Relationship between them was analyzed. No significant difference in the expression of CD11a between the angina and control groups was obsd. The expression of CD11b on lymphocytes and neutrophilic granulocytes in the unstable angina group and stable angina group was significantly higher than that in the healthy people (P 0.05). The amt. of adhesion mol. related protein CD11b on neutrophilic granulocytes and lymphocytes in patients with unstable angina was higher than that in patients with stable angina and control group, but there was no relationship between the expression level of CD11b and degree of coronary stenosis.
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Xenogeneic homologous genes, molecular evolution and cancer therapy. Tian, Ling; Wei, Yuquan. Center for Biotherapy of Cancer and Cancer Research Center, First University Hospital, West China Medical Center, Sichuan University, Chengdu, Peop. Rep. China. Progress in Natural Science (2001), 11(12), 893-904. Publisher: Science in China Press, CODEN: PNASEA ISSN: 1002-0071. Journal; General Review written in English. CAN 137:4552 AN 2002:17265 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A review which discusses cancer biotherapy with xenogeneic homologous genes. Cancer is one of the main causes for death of human beings to date, and cancer biotherapy (mainly immunotherapy and gene therapy) has become the most promising approach after surgical therapy, radiotherapy and chemotherapy. However, there are still many limitations on cancer immunotherapy and gene therapy; therefore great effort is being made to develop new strategies. It has been known that, in the process of evolution, a no. of genes, the so-called xenogeneic homologous genes, are well-conserved and show the structural and/or functional similarity between various species to some degree. The nucleotide changes between various xenogeneic homologous genes are derived from mutation, and most of them are neutral mutations. Considering that the subtle differences in xenogeneic homologous genes can break immune tolerance, enhance the immunogenicity and induce autologous immune response to eliminate tumor cells, the authors expect that a strategy of inducing autoimmune response using the property of xenogeneic homologous genes will become a new therapy for cancer. Moreover, this therapy can also be used in the treatment of other diseases, such as autoimmune diseases and AIDS. This article will discuss the xenogeneic homologous genes, mol. evolution and cancer therapy.
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Anti-tumor immune response against mouse melanoma to xenogeneic vaccination. Luo, Feng; Wei, Yuquan; Bing, Kan; Yang, Li; Tian, Ling; Lu, You; Peng, Feng; Jiang, Yu; Liu, Jiyan; Zhao, Xia; Zou, Liqun; Lei, Song; Mao, Yongqiu. Center of Cancer Biotherapy, First Affiliated Hospital, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Zhonghua Zhongliu Zazhi (2001), 23(2), 118-121. Publisher: Zhongguo Yixue Kexueyuan Zhongliu Yanjiuso, Zhongliu Yiyuan, CODEN: CCLCDY ISSN: 0253-3766. Journal written in Chinese. CAN 136:149325 AN 2001:387657 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The inhibition of melanoma growth in mice by vaccination with xenogeneic melanocytes was studied. Xenogeneic vaccine was prepd. from pig eye melanocytes. It was used before or after B16 melanoma challenge in C57 mice. The size of tumor was monitored. Cytotoxic T lymphocyte (CTL) activity of mouse spleen cells was measured by 51Cr release assay. Antibody response against pig melanocytes and B16 melanoma cells were detected by indirect ELISA. Preventive vaccination resulted in inhibition of tumor growth in 90% of the immunized mice, while therapeutic vaccination inhibited tumor growth in 50% of the treated mice. Specific CTL activity and antibodies in the immunized mice were detected. Anti-tumor immune response capable of inhibiting melanoma growth can be induced by xenogeneic melanocyte vaccination.
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Effects of calcium channel antagonist on gastrin-induced proliferation of HT29 colon carcinoma cells. Wu, Hao; Zhang, Zheng; Huang, Xinglan; Wei, Yuquan; Lei, Song. Department of Internal Medicine, The First Affiliated Hospital, WCUMS, Chengdu, Peop. Rep. China. Huaxi Yike Daxue Xuebao (2001), 32(1), 96-97, 122. Publisher: Huaxi Yike Daxue, CODEN: HYDXET ISSN: 0257-7712. Journal written in Chinese. CAN 136:3744 AN 2001:328292 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Pentagastrin (2.5 x 10-6 mol/L) induced a rapid increase of intracellular free calcium ([Ca2+]i) and proliferation of in HT29 colon carcinoma cells. Nifedipine significantly inhibited the pentagastrin-induced increase of [Ca2+]i and cell proliferation. Apparently, nifedipine may block the influx of Ca2+ and inhibit the pentagastrin-induced tumor cell proliferation.
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Recombinant toxin adenovirus mutant for treating cancer gene HER-2/neu overexpressed tumor. Wei, Yuquan; Tian, Ling. (West-China Medical Univ., Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu (2000), 15 pp. CODEN: CNXXEV CN 1258737 A 20000705 Patent written in Chinese. Application: CN 98-124027 19981225. Priority: . CAN 134:231856 AN 2001:249891 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Patent Family Information
Patent No. Kind Date Application No. Date
CN 1258737 A 20000705 CN 1998-124027 19981225
Priority Application
CN 1998-124027 19981225
Abstract
The process comprises amplifying Ela gene fragment from plasmid pXCl by PCR, digesting with Nhe I/Spe I to obtain Ela gene; digesting plasmid pRL-CMV with Nhe I/Xba I to obtain vector pRL- CMV, connecting with Ela gene to obtain expression vector pRL-Ela; digesting with BamH I/Bgl II to obtain expression element of Ela, digesting plasmid pdElsplA with BamH I/Bgl II to obtain vector pdElsplA, connecting with expression element of Ela to obtain plasmid pElasplA of adenovirus precursor; co-transfecting with plasmid pBHG-PE and pElasplA into 293 cell, screening, and identifying. The plasmid pXCl contains early-transcription region of type-5 adenovirus (Ad5). The plasmid pRL-CMV contains luciferase gene, regulation sequence for eukaryotic gene, immediate early initiator and enhancer of CMV gene, intron, SV40, and polyadenylic acid.
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Progress in targeting gene therapy. Xie, Xingjiang; Tian, Ling; Wei, Yuquan. Cancer Center, the First Affiliated Hospital, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Zhonghua Yixue Yichuanxue Zazhi (2001), 18(1), 59-61. Publisher: Huaxi Yike Daxue, CODEN: ZYXZER ISSN: 1003-9406. Journal; General Review written in Chinese. CAN 135:251135 AN 2001:234898 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A review with 12 refs. on progress in targeting gene therapy. Many new technologies show great promising prospects such as the delivery of therapeutic gene to targeted cells by some antibodies or ligands, the use of specific gene promoter to tightly control gene expression within disease microenvironment, the use of some orally taken drugs for regulating expression time and expression level of DNA, and the targeted correction of deleted or mutated gene using RNA/DNA oligonucleotides.
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Angiogenesis inhibitor-endostatin and preparation process thereof. Wei, Yuquan; Yang, Li. (Huaxi Medical Univ., Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu (2000), 28 pp. CODEN: CNXXEV CN 1266064 A 20000913 Patent written in Chinese. Application: CN 99-114693 19990303. Priority: . CAN 134:233359 AN 2001:231223 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Patent Family Information
Patent No. Kind Date Application No. Date
CN 1266064 A 20000913 CN 1999-114693 19990303
Priority Application
CN 1999-114693 19990303
Abstract
Methods of expression and prepn. of angiogenesis inhibitor-endostatin are disclosed. Endostatin is the C-terminal proteolytic fragment of human 1336-amino acid 1 type XVIII collagen corresponding to residues from 1,154 to 1,336, which capable of inhibiting endothelial cell proliferation, angiogenesis and tumor growth. The cDNA for human endostatin is cloned from human tissues (liver, lung, or kidney) by RT-PCR and methods to construct its prokaryote and eukaryote expression vectors, and isolate it and purify it (through reverse column chromatog.) are also provided. The biol. activity of purified endostatin is also tested for cancer treatment in vitro and in vivo.
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Recombinant adenovirus and its use for treating tumor suppressor p53 deficient cancers. Wei, Yuquan; Tian, Ling. (West China Medical University, Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu (2000), 14 pp. CODEN: CNXXEV CN 1258742 A 20000705 Patent written in Chinese. Application: CN 98-124026 19981225. Priority: . CAN 134:111232 AN 2001:97074 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Patent Family Information
Patent No. Kind Date Application No. Date
CN 1258742 A 20000705 CN 1998-124026 19981225
Priority Application
CN 1998-124026 19981225
Abstract
A recombinant adenovirus CBC-015 is prepd. and isolated by constructing adenovirus plasmid vector pAd-E1b and transfecting it into 293 cell. This recombinant adenovirus is tested by PCR and maybe useful for tumor suppressor p53 deficient cancers.
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Change of apoptotic status in the human colorectal adenoma-carcinoma sequences and its correlation with carcinogenesis and prognosis. Li, Li; Yan, Lunan; Wang, Zhong; Liu, Zhanpei; Wei, Yuquan; Huang, Guangqi. Department of General Surgery, First Affiliated Hospital, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Chinese Medical Journal (Beijing, English Edition) (2000), 113(10), 886-888. Publisher: Chinese Medical Association, CODEN: CMJODS ISSN: 0366-6999. Journal written in English. CAN 134:220578 AN 2000:775262 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The objective was to assess apoptotic status during the development of colorectal cancer and its prognostic value. The apoptotic frequency of 168 fresh adenocarcinoma specimens and primary cultured cells at 2, 12, 24 and 48 h (9 normal mucosa, 4 adenomas and 9 adenocarcinomas) were measured by flow cytometry (FCM). Apoptotic indexes (AI) in situ for 25 adenomas and 77 adenocarcinomas were visualized by TdT-mediated dUTP nick end labeling (TUNEL), Ki-s5 labeling indexes, (KI), bcl-2, bax, waf1 and p53 were immunostained with ABC method. The culture-related apoptosis at 24-48 h in vitro was obviously decreased in cultured tumor cells when compared with mucosa cells. Spontaneous apoptosis in situ occurred more frequently in tumor with aneuploid type at late stage. There was pos. relationship between apoptosis and proliferative activity, detd. by both TUNEL and FCM methods. The well-differentiated or early stage lesions with intensive bcl-2/bax expression were significantly more likely to have low AI. P53 accumulation and waf1 depression were mainly related to KI, whereas bax and waf1 overexpression led to a comparatively higher AI/KI ratio. Bcl-2 and KI were found to be independent risk factors. The data suggest that the depressed susceptibility to inductive apoptosis may contribute to the initial phase of tumorigenesis, and spontaneous apoptosis in vivo may serve as a marker of tumor progression. The bcl-2 and KI may be valuable in predicting prognosis in colorectal cancer.
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Advances in the researches of DNA vaccines against tumors. Lu, You; Tian, Ling; Wei, Yuquan; Yang, Li; Zhao, Xiao. Center of Biotherapy for Cancer, the First Affiliated Hospital, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Zhonghua Yixue Yichuanxue Zazhi (2000), 17(4), 288-290. Publisher: Huaxi Yike Daxue, CODEN: ZYXZER ISSN: 1003-9406. Journal; General Review written in Chinese. CAN 134:235732 AN 2000:673705 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
A review with 20 refs. The injection of naked plasmid DNA directly into the muscle cells of hosts has been shown to induce potent immune responses, as well as to express large amts. of gene product; this has provided a new way of treatment for tumor. The past ten years have witnessed tremendous growth in the field of gene therapy for cancer using i.m. injection of plasmid DNA. Many studies have suggested that the immunostimulatory DNA sequences (ISS) in vector backbone of plasmid DNA, delivering adjuvant and mitogenic activity, are necessary for effective intradermal gene immunization. It has been postulated that muscle cells serve as a reservoir of expressed antigen with subsequent transfer to bone marrow-derived APCs. Thus, immunization with plasmid DNA can trigger strong and persistent cell-mediated and humoral immune responses to the antigen encoded by the plasmid. Increasing evidence demonstrates that DNA immunization can prevent or inhibit tumor development. In this review, the present authors presented a discussion on the characteristics of immune response to DNA vaccines and a summary of the effects of immune response against tumors.
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Quantification analysis of vascular endothelial cells in human solid tumor. Jiang, Yu; Wei, Yuquan; Lei, Song; Mao, Yongqiu; Kan, Bing; Peng, Feng. Center for Biotherpay of Cancer, Center of Onocology, The First Affiliated Hospital, WCUMS, Chengdu, Peop. Rep. China. Huaxi Yike Daxue Xuebao (2000), 31(2), 233-235, 238. Publisher: Huaxi Yike Daxue, CODEN: HYDXET ISSN: 0257-7712. Journal written in Chinese. CAN 133:234690 AN 2000:556234 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Angiogenesis is essential for the growth and metastasis of solid tumors. Intratumoral microvessel count, which represents a measure of tumor angiogenesis, has been assocd. with prognosis of patients with a variety of malignancies. But till now, it is still a problem to quantitate the angiogenesis of tumors exactly. In this study, the endothelial cells in 20 solid tumors were conjugated with anti-CD34 monoclonal antibodies labeled with red-fluorescent substances, and were obsd. by fluorescence microscopy and quantitated by flow cytometry. The results of flow cytometry were analyzed by t-test. The correlation was analyzed between percentage of flow cytometry and vessel counts (light microscopy, 200) in each tumor which was stained with anti-CD34 monoclonal antibodies and anti-VIII factor related antigen polyclonal antibodies by using immunohistochem. The resulted showed there was significant difference between the treatment group and control group in flow cytometry, and a significant correlation between the endothelial cells percentage by flow cytometry and the vessel counts by immunohistochem. was obsd., although the av. vessel counts were approx. three times with CD34 staining as much as with VIII factor related antigens staining. These data indicate that flows by cytometry as a novel method can quantitate angiogenesis of tumors exactly and quickly.
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The relationship of cellular DNA content with clinical stage and biological features of colorectal cancer. Zou, Liqun; Mao, Yongqiu; Lei, Song; Wei, Yuquan; Zhao, Xia; Bing, Kan; Jiang, Yu; Peng, Feng; Wang, Qingu; Tian, Ling; Yang, Li; Liu, Jiyan. Department of Biotherapy for Cancer, The First Affiliated Hospital, WCUMS, Chengdu, Peop. Rep. China. Huaxi Yike Daxue Xuebao (2000), 31(2), 180-182. Publisher: Huaxi Yike Daxue, CODEN: HYDXET ISSN: 0257-7712. Journal written in Chinese. CAN 134:84177 AN 2000:556091 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The purpose of this study was to explore the relationship of cellular DNA content with clin. stage and biol. features of cancer. Flow cytometry was performed on fresh specimens from 86 patients from 1997 to 1998. Forty-five (53.3%) specimens were found to contain cells with abnormal DNA (DNA nondiploid). Although none of the sex, age, site, differentiation variables correlated with flow cytometric DNA ploidy, nondiploidy was assocd. with Dukes' stage and lymph node metastasis. Duke's A stage tumors were more frequently diploid than were more advanced tumors, but no difference between Duke's B, C and D stages were obsd. These findings suggest that ploidy is assocd. with some pathol. factors.
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Sulindac-induced apoptosis of HT-29 colon adenocarcinoma cells. Yang, Zhengbin; Ouyang, Qin; Wei, Yuquan. Department of Gastroenterology, The First Affiliated Hospital, WCUMS, Chengdu, Peop. Rep. China. Huaxi Yike Daxue Xuebao (2000), 31(2), 177-179. Publisher: Huaxi Yike Daxue, CODEN: HYDXET ISSN: 0257-7712. Journal written in Chinese. CAN 133:329211 AN 2000:556087 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Flow cytometry, transmission electron microscopy and DNA electrophoresis all demonstrated that sulindac could induce apoptosis of HT-29 colon adenocarcinoma cells in a time- and concn.-dependent manner. After treatment with 0.3, 0.6 and 1.2 mM sulindac for 48 h, the percentages of apoptotic cells were 5.8%, 7.6% and 11.7%, resp., vs. 2.9% in controls; after treatment for 72 h, the percentages had increased to 12.5%, 15.4% and 24.4%, resp., vs. 5.1% in controls. Thus, apoptosis in the colon adenocarcinoma HT-29 cell line can be induced by sulindac.
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The significance of p27 expression and DNA content in benign and malignant bone tumors. Zeng, Jiancheng; Hu, Yuzhuo; Pei, Fuxing; Lei, Song; Mao, Yingqiu; Wei, Yuquan. Department of Orthopedics, First affiliated Hospital of Huasi Medical University, Chengdu, Peop. Rep. China. Zhongguo Zhongliu Linchang (2000), 27(3), 180-183. Publisher: Zhongguo Zhongliu Linchang Bianji Weiyuanhui, CODEN: ZZLIEP ISSN: 1000-8179. Journal written in Chinese. CAN 134:40238 AN 2000:523644 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Objective: To explore the relationship between p27 expression and DNA content in benign and malignant bone tumors and its clin. significance. Methods: The expression of p27, S-phase fraction (SPF) and DNA content in 52 fresh specimens of bone tumors confirmed by pathol. (osteochondroma 10, giant cell tumor of bone 10, osteosarcoma 20 and other malignant bone tumors 12) were measured by flow cytometry and quant. immunofluorescence and the correlation of them was analyzed. Results: The mean fluorescence index (FI) of p27 protein in 52 cases was 1.37 (osteochondroma 2.24 0.38, giant cell tumor of bone 1.79 0.36, osteosarcoma 1.06 0.39 and other malignant tumors 1.01 0.45). Twenty-five cases with p27 lower than 1.37 were all malignant bone tumors. DNA index (DI) and SPF in the group with p27 lower than 1.37 were far higher than that of the group with p27 higher than or equal to 1.37 (P<0.001). Conclusion: We suggest that low expression of p27 protein may lead to abnormality of cell cycle, while increase of S-phase cells, synthesis of DNA and hyperproliferation and malignant change of cells would be one of the causes of tumorigenesis of bone tumor.
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Increase in thermosensitivity of cervical cancer and ovarian cancer cells by HSP70 antisense oligodeoxynucleotides. Zhao, Xia; Wei, Yuquan. Department of Obstetrics and Gynecology, Second Affiliated Hospital, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Zhonghua Zhongliu Zazhi (2000), 22(2), 99-101. Publisher: Zhongguo Yixue Kexueyuan Zhongliu Yanjiuso, Zhongliu Yiyuan, CODEN: CCLCDY ISSN: 0253-3766. Journal written in Chinese. CAN 133:279665 AN 2000:249191 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The thermosensitizing effect of heat shock protein 70 (HSP70) antisense oligodeoxynucleotides (ODN) on cervical and ovarian cancer cells was studied. Cervical cancer cell line Hela and ovarian cancer cell line MCAS were used to study their sensitivity to heat (42, 1 h) treatment. Heat-induced changes in cell viability was assessed by colony formation assay and apoptosis by cell morphol., agarose gel electrophoresis and flow cytometry. Thermosensitivity of Hela and MCAS cells pretreated with HSP70 antisense ODN increased as compared to cells treated with heat alone. The increase was antisense dose dependent. The percentage of apoptotic cells increased significantly when both heat and antisense ODN were applied although heat treatment or antisense ODN did induce apoptosis of HeLa and MCAS cells. The effect was dependent on the dose of HSP70 antisense ODN. The thermosensitivity of ovarian cancer cells and cervical cancer cells can be increased by HSP70 antisense oligomer. Apoptosis may be involved in the increase in thermosensitivity.
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Induction of apoptosis in ovarian carcinoma cells by HSP 70 antisense oligodeoxynucleotides. Zhao, Xia; Wei, Yuquan; Peng, Zitan. Second Affiliated Hospital, West China University of Medical Sciences Chengdu, Sichuan, Peop. Rep. China. Zhonghua Yixue Yichuanxue Zazhi (2000), 17(1), 32-35. Publisher: Huaxi Yike Daxue, CODEN: ZYXZER ISSN: 1003-9406. Journal written in Chinese. CAN 133:129573 AN 2000:198876 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Objective: To investigate the role of HSP 70 in the proliferation and survival of ovarian carcinoma cells by inhibiting HSP 70 expression with HSP 70 antisense oligomer. Methods: Morphol. changes of apoptotic cells were investigated by light microscopy. DNA fragmentation was analyzed by agarose gel electrophoresis. Kinetics of induction of apoptosis and cell cycle were analyzed by flow cytometry. Results: The HSP 70 antisense oligomer treated ovarian carcinoma cells showed apparent inhibition of proliferation and characteristic morphol. changes of apoptosis. Also, a ladder-like pattern of DNA fragments was demonstrated on agarose gel electrophoresis. HSP 70 antisense-oligomer induced apoptosis of ovarian carcinoma cells mainly in G1/S phase in 8.3% to 41% at 1 to 20mol/L. The apoptosis-inducting effect of HSP 70 antisense oligomer was in a dose- and time-dependent manner. Conclusion: HSP 70 antisense oligomer could not only inhibit the proliferation but also induce the apoptosis in ovarian carcinoma cells.
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Changes of F-actin in neutrophils under fluid shear stress. Tian, Wei; Chen, Huaiqing; Chen, Yunying; Lei, Song; Chen, Yunshuang; Bu, Hong; Wei, Yuquan. Institute of Biomedical Engineering, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Shengwu Yixue Gongchengxue Zazhi (1999), 16(4), 399-405. Publisher: Shengwu Yixue Gongchengxue Zazhi, CODEN: SYGZF2 ISSN: 1001-5515. Journal written in Chinese. CAN 132:234819 AN 2000:84027 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Neutrophils are the main defender in the body, and their physiol. characters are assocd. with functions. Because the environments of neutrophils maturation and storage are different from their function environment, they have to adapt these changes. Filamentous actin (F-actin), as one of the important cytoskeleton components of neutrophils, has different quantity and distribution under different physiol. conditions. Previous researches are all focused on static activation of neutrophils using various stimulants such as formyl-methionyl-leucyl-phenylalanine (f-MLP) and tumor necrosis factor(TNF). In our study, Low-Shear 30 and NXE-1 rheometers were used to provide steady or sinusoidal-oscillatory fluid shear stress on sepd. neutrophils. Fluorescent agent Trite-Phalloidin was added to label F-actin and the mean fluorescent intensity tested by flow cytometry was used as the indication of F-actin quantity and confocal laser scan microscope was used to detect the distribution of F-actin in neutrophils. We found that F-actin polymn. was significantly decreased under both steady and sinusoidal-oscillatory shear stress when the shear stress was below certain level. The d. of the cortex F-actin near cell membrane in cells sheared became thinner than in that without shear stress. F-MLP and TNF both could increase actin polymn. in neutrophils due to activation. When neutrophils were activated with f-MLP or TNF under shear stress, the quantity of F-actin in the cells was also decreased, but it was still higher than that in the cells absent of f-MLP or TNF. We concluded that neutrophils had different mech. properties in different fluid environments. F-actin content and distribution would change according to the shear stress values to play their determinant role in neutrophils mech. adaptation. The mechanism of actin polymn. in neutrophils for mech. action is probably different from that for chemoattractants and cytokine activation.
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Study on synthesis MTX-D conjugate and its sensitivity to K562 cells. Yan, Zhongqin; Zhong, Yuguo; Wei, Yuquan. School of Pharmacy, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Huaxi Yaoxue Zazhi (1999), 14(5-6), 302-305. Publisher: Huaxi Yike Daxue Yaoxueyuan, CODEN: HYZAE2 ISSN: 1006-0103. Journal written in Chinese. CAN 132:208022 AN 1999:804952 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The title compd. tested with apoptosis fluorescence straining (AFS) assay was prepd. from dextran (T-77) with cyanogen bromide followed by reacting with 6-aminocaproic acid as a spacer and conjugated with methotrexate (MTX) in the presence of carbodiimide (EDC) as catalyst. MTX-D being sensitivity to K562 cells was able to induce apoptosis of tumor cells, and to affect growth period of the cells.
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Effects of fluid shear stress on neutrophil surface expression of adhesion molecules. Chen, Huaiqing; Tian, Wei; Lei, Song; Wei, Yuquan; Chen, Yunshuang. Institute of Biomedical Engineering, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Shengwu Yixue Gongchengxue Zazhi (1999), 16(3), 288-294. Publisher: Shengwu Yixue Gongchengxue Zazhi, CODEN: SYGZF2 ISSN: 1001-5515. Journal written in Chinese. CAN 132:179513 AN 1999:717305 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Fluid shear is a physiol. and functional environment for circulating neutrophils where various chemoattractants and cytokines have different effects on neutrophils, activating them or evoking inflammation. Recently, we used Low-Shear 30 to provide steady or sinusoidal-oscillated fluid shear stress on sepd. neutrophils with or without f-MLP/TNF stimulation. FITC-anti CD18 monoclonal antibody and PE-anti CD62L monoclonal antibody were added to label CD18 and CD62L on neutrophils surface resp. Flow cytometry was used to quantify surface expression and pos. cell percentage of these two adhesion mols. Fluid shear stress increases the expression of CD18 and decreases the CD62L pos. cell percentage, but it does not influence the CD62L expression on the CD62L pos. cells. The effects of the different patterns of fluid shear stress are not much different, f-MLP or TNF can slightly decrease the expression of CD18 and make CD62L shedding, resp. When one of the two activators and the fluid shear stress act together, the expression of the two adherent mols. can be added. The mech. and biochem. stimulative action can produce different effects through different receptors and different signal transduction pathways. The mech. environment should be considered in the research of activation and adhesion of neutrophils.
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An analysis of DNA content in human tumor by flow cytometry. Lei, Song; Wei, Yuquan; Mao, Yongqiu; Hang, Zhenbiao; Zhao, Xia; Yan, Lunan. WCUMS, The First Affiliated Hospital, Chengdu, Peop. Rep. China. Huaxi Yike Daxue Xuebao (1999), 30(3), 324-326. Publisher: Huaxi Yike Daxue, CODEN: HYDXET ISSN: 0257-7712. Journal written in Chinese. CAN 132:148621 AN 1999:657588 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
This study was intended to evaluate the relationship between the DNA content of cell cycle and the histol. in human tumors. We detected the DNA content in 405 cases of fresh human tumor tissue by means of flow cytometry and obsd. the histol. of tumor with light microscopy. The occurrences of aneuploidy in 22 cases of benign tumor and 383 cases of malignant tumor were 27% and 52% resp. There were differences in aneuploidy in different histol. types of tumor. The aneuploidy in adenocarcinoma was about 50%, that in sarcoma was more than 37%, and that in squamous carcinoma less than 17%. S10, G2/m10 or S20, G2/m5 were present in malignant tumors, but were not found in benign tumors. The results suggest that there is obvious difference in the occurrences of aneuploidy in benign and malignant tumors and in different histol. type of tumor, (P<0. 05). Benign and malignant tumor may be distinguished when SPF and G2/m reach a higher level (P<0. 01).
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Leukocyte adhesion molecule expression and circulating ICAM-1, E-selectin levels during cardiopulmonary bypass in patients undergoing valve replacement. Xiao, Xijun; Zhang, Chongjie; Wei, Yuquan; Zhang, Ping; Zhao, Feng; Wang, Jun; Tao, Ping; Tian, Zipiao. The First Affiliated Hospital, WCUMS, Chengdu, Peop. Rep. China. Huaxi Yike Daxue Xuebao (1999), 30(1), 81-84. Publisher: Huaxi Yike Daxue, CODEN: HYDXET ISSN: 0257-7712. Journal written in Chinese. CAN 131:72056 AN 1999:252948 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The objectives of this study were to measure leukocyte adhesion mol. expression, assess the possibility of evaluating the serum levels of the sol. form of endothelium adhesion mol. as an alternative for endothelia cell activation. and analyze the correlation and regression between leukocyte adhesion mol. expression and sol. endothelium adhesion mol. levels during cardiopulmonary bypass (CPB) in patients undergoing valve replacement. Twelve adult patients were included in this study. The extracorporeal circuit was primed with Ringer's lactate. After systemic heparinization (300IU/kg), CPB was initiated with a bubble oxygenator. Patients were cooled to about 28C,at that temp., aorta was clamped and cold crystalloid cardioplegia was delivered into aortic root. The activatal clotting time was maintained at greater than 450 s. 1-1. 3 Mg of protamine was administered for each 100 IU of heparin given prior to CPB. Peripheral blood was collected at the day prior to surgery and the morning of postoperative days 1,2,5, and 7 for detn. of the expression of leukocyte CD11a, CD18,CD11a/CD18,CD11b,CD11b/CD18 (immunofluorescent flow cytometry) and the plasma sol. intercellular adhesion mol.-1 (sICAM-1), sol. E-selectin (sE-selectin) (enzyme-linked immunoabsorbent assay). CPB caused a sustained increase in the leukocyte CD11a, CD18, CD11a/CD18, CD11b/CD18 expression and the plasma sICAM-1, sE-selectin levels in patients (P<0. 05). Upregulation of the leukocyte adhesion mol. expression and increase of the circulation endothelium adhesion mol. levels lasted at least 2 days after surgery (P<0. 05) except CD11a. There were correlation and regression between leukocyte adhesion mol. expression and sol. endothelium adhesion mol. levels (r=0.91-0.99, P=0.0322-0.0005; Y=-1377.06-44.64 + 1.49-14.78a to Y=44.64+1.49a) These results suggest that CPB causes obvious leukocyte-endothelia interactions, that the expression of these adhesion mols.
are presented by means of receptor-ligand form, and that the measurement of sICAM-1 and sE-selectin may well be used as a marker of endothelial cell activation.
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Effect of vasectomy on Bcl-2 and Bax protein expression in spermatogenic cells of male rat. Shen, Kai; Yang, Yuru; Li, Hong; Shen, Hong; Zhang, Hongying; Wei, Yuquan; Tang, Xiaoda; Huang, Mingkong. Department of Urology, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Shengzhi Yu Biyun (1999), 19(1), 11-16. Publisher: Shengzhi Yu Biyun Bianjibu, CODEN: SCYYDZ ISSN: 0253-357X. Journal written in Chinese. CAN 131:16912 AN 1999:194524 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
Immunohistochem. method was used to detect the expression of Bcl-2 and Bax protein in the testis of vasectomized and sham-operated adult rats during and at the end of the 12th postoperative week. The results showed that the total no. of Bcl-2 pos. stained cells after vasectomy declined. The no. of Bcl-2 pos. stained cells, on an av., was 45.114.9 and 71.315.7 sep. in each 300 spermatogonia and each 300 spermatocytes in vasectomized rats while in the sham-operated group, the no. was 52.712.1 and 81.114.3 sep. The no. of Bcl-2 pos. cells in vasectomized group decreased (P <0.05). The no. of Bax pos. cells in each 300 spermatogonia and each 300 spermatocytes was 84.219.4 and 49.917.3 resp. after vasectomy, while in the sham-operated group, the no. was 65.917.0 and 42.917.3 resp., the no. of Bax pos. cells increased significantly (P <0.05). These findings show that vasectomy may decrease the expression of Bcl-2 protein and increase the expression of Bax protein, therefore enhance apoptosis of spermatogenic cells after vasectomy.
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Apoptosis of ovarian carcinoma cell line induced by amiloride. Zhao, Xia; Wei, Yuquan; Peng, Zhilan. Department of Obstetrics and Gynecology, Second University Hospital, West China University of Medical Sciences, Chengdu, Peop. Rep. China. Zhonghua Zhongliu Zazhi (1999), 21(1), 22-24. Publisher: Zhongguo Yixue Kexueyuan Zhongliu Yanjiuso, Zhongliu Yiyuan, CODEN: CCLCDY ISSN: 0253-3766. Journal written in Chinese. CAN 131:39307 AN 1999:181827 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The induction of apoptosis and its possible mechanism in amiloride-treated ovarian carcinoma cell line were studied. Morphol. changes of apoptotic cells were obsd. by light and fluorescence microscopy. DNA fragmentation was analyzed by agarose gel electrophoresis. The no. of hypodiploid cells (apoptotic cells) was quant. assessed by flow cytometry and intracellular pH was also analyzed. Amiloride-treated ovarian carcinoma cells showed morphol. characteristics of apoptosis. A ladder-like pattern of DNA fragmentation was shown on agarose gel electrophoresis. Amiloride at 0.01-5 mol L-1 could induce apoptosis in 18.7%-61.6% of ovarian carcinoma cells. The apoptosis-inducing effect of amiloride was dose- and time-dependent. Amiloride induced intracellular acidification in a subpopulation of the treated cells. These isolated acidified cells showed chromatin condensation as well as DNA degrdn. with characteristics of apoptosis. There was correlation between apoptotic cells and acidic cells. Amiloride triggers apoptosis of ovarian carcinoma cells and intracellular acidification may be involved in the mechanism of apoptosis.
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Effect of Ox-LDL on cell cycling phases and PCNA, P53, P27 and c-erbB-2 expression in cultured human arterial smooth muscle cells. Li, Zaiquan; Liu, Bingwen; Wei, Yuquan. Apolipoprotein Res. Unit, Inst. Biochem. Mol. Biol., Chengdu, Peop. Rep. China. Huaxi Yike Daxue Xuebao (1998), 29(4), 394-398. Publisher: Huaxi Yike Daxue, CODEN: HYDXET ISSN: 0257-7712. Journal written in Chinese. CAN 130:207809 AN 1999:57490 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
It was found in our previous study that oxidative modification LDL (Ox-LDL) could stimulate the proliferation of cultured human arterial smooth muscle cells (SMC). Yet, the mechanism responsible for the SMC proliferation induced by Ox-LDL is not clear. Proliferating cell nuclear antigen (PCNA), P53 and P27 are the key regulatory factors of cell replication. In order to observe the effects of Ox-LDL on cell cycling phase and PCNA, P53, P27 and c-erb B-2 expression in SMC, we used the flow cytometric method in the present study on the proliferation of cultured human SMC induced by OX-LDL. The results showed a relation between the Ox-LDL mediated SMC proliferation and the cycling phase shifting. The relative no. of S phase cells in the Ox-LDL group was higher than that of the control group (22.9% vs 15.7%). Ox-LDL mediated SMC proliferation was accompanied with the increasing expression of PCNA. The percentage of specific PCNA pos. FITC cells on the Ox-LDL group was significantly higher than that of the control group (12.6% vs. 6.5%). PMA, an activator of protein kinase C (PKC), stimulated SMC proliferation and increased the PCNA expression in cultured SMC, while the PKC inhibitor, F109203X, significantly decreased the PCNA expression in SMC (PCNA pos. cells 13.4% vs 0.4%). No changes were obsd. in the expression of P53, P27 and c-erb -2 in the cultured proliferating SMC induced by Ox-LDL. In all, the results suggest that the Ox-LDL mediated SMC proliferation is related to increasing S Phase cells and involved in the PCNA expression which might undergo the PKC cellular signal transduction pathway.
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Detection of expression of the multidrug resistance gene product (P170) in human tumor tissues and cells by flow cytometry. Bing, Kan; Luo, Feng; Lei, Song; Mao, Yongqiu; Zou, Liqun; Zhao, Xia; Wei, Yuquan. Center Biotherapy Cancer, First Affiliated Hospital, Chengdu, Peop. Rep. China. Huaxi Yike Daxue Xuebao (1998), 29(3), 269-271. Publisher: Huaxi Yike Daxue, CODEN: HYDXET ISSN: 0257-7712. Journal written in Chinese. CAN 130:162840 AN 1998:767547 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
We have detected the human multidrug resistance gene (MDR1) product P170 in 29 solid tumor samples and K562 cell line through indirect immunofluorescence staining by flow cytometry using mouse monoclonal antibody (McAb). The results showed that the expression of P170 was detected in 18 samples, the pos. ratio being 62.1%; the expression was not detected in 11 samples, the neg. ratio being 37.9%; and 99.9% of K562 cells expressed P170. In 16 of the 18 pos. samples, the percent ratio of tumor cells for expression of P170 was less than 30%; in the other 2, more than 30%. This indicated that the pos. ratio of P170 of most tumor samples was high, but their percent ratio of P170 was low. Thus it provided a parameter for ref. in evaluating the efficacy of clin. antitumor treatments.
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Induction of antitumor immune response by NK-cell-sensitive target cells transfected by B7-1 gene. Zhao, Xia; Wei, Yuquan; Kariya, Yoshitaka. Department Obstetrics Gynecology, Second Affiliated Hospital, West China University Medical Sciences, Chengdu, Peop. Rep. China. Zhonghua Yixue Yichuanxue Zazhi (1998), 15(4), 210-213. Publisher: Huaxi Yike Daxue, CODEN: ZYXZER ISSN: 1003-9406. Journal written in Chinese. CAN 130:65153 AN 1998:636085 CAPLUS (Copyright (C) 2006 ACS on SciFinder (R))
Abstract
The authors investigated whether the expression of B7-1 is involved in NK cell activation. The B7-1 gene was transfected into the K562 cell line (a sensitive target cell of NK cell, without MHC class I expression) by electroporation. Specific cytotoxicity of NK cells to B7+K562 cell was assayed by 4-h Cr release assay. The proliferation of NK cells induced by B7+K562 cell was analyzed by Flow Cytometry. Expansion of NK cells and cloning of NK cells were preformed by mixed lymphocyte-tumor cultures and limiting diln. anal. Prodn. of TNF-, GM-CSF and IFN- was detected by ELISA tests. The cytotoxicity of NK cells to B7+K562 cell could be induced and the no. of NK cells could be increased by simulation with B7+K562 cells, in particular, further enhanced with biotherapeutic agent such as OK432 and IL-2. Also, NK cell clones were established. Cytokines such as TNF-, INF- and GM-CSF were detected in the supernatant produced by NK cell clones. In addn., culture of lymphocytes in the presence of supernatant produced by NK cell clones and tumor antigens resulted in an increased autologous tumor killing (ATK). These findings suggest that K562 cells transfected by B7-1 gene may elicit a series of antitumor immunity and these cells may be used for further development of therapeutic tumor vaccine.
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Study on the expression of HSP70 and HSP90beta in nasopharyngeal carcinoma and the clinical significance. Liu Tao; Liang Chuanyu; Tian Ling; Wei Yuquan; Zhao Jumei Department of Otorhinolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology (2005), 19(14), 640-2, 645. Journal code: 9426080. ISSN:1001-1781. Journal; Article; (JOURNAL ARTICLE) written in Chinese. PubMed ID 16248461 AN 2005570926 In-process for MEDLINE (Copyright (C) 2006 U.S. National Library of Medicine on SciFinder (R))
Abstract
OBJECTIVE: To study the expression of HSP70 and HSP90beta and its clinical significance in human nasopharyngeal carcinom. METHOD: Immunohistochemical method SP was used to detect the expression of HSP70 and HSP90beta in 50 cases and its clinical significance were studied. RESULT: The positive rate of HSP70 and HSP90beta were 72% and 56% respectively. Analysis of patients, survival demonstrated that the prognosis of NPC with HSP70 (-) and HSP90beta (-) expression were significantly better than the others. CONCLUSION: The overexpression of HSP70 and HSP90beta were probably concerned with the occurrence, development and prognosis of nasopharyngeal carcinoma.
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Receptor selection and B cell immune tolerance. Hu Bing; Wei Yuquan Key Laboratory of Biotherapy of Human Diseases, Ministry of Education, People's Republic of China Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi (2005), 22(2), 374-6. Journal code: 9426398. ISSN:1001-5515. Journal; Article; (JOURNAL ARTICLE); General Review; (REVIEW) written in Chinese. PubMed ID 15884558 AN 2005246124 MEDLINE (Copyright (C) 2006 U.S. National Library of Medicine on SciFinder (R))
Abstract
The immune system of mammalian has developed sophisticated mechanism to deal with diverse unknown antigens by random rearrangement of V, D and J antigen gene fragments. The immune self-tolerance is the mechanism to avoid self-destruction by the gene rearrangement generated autoreactive lymphocytes. Until recently it was believed that autoreactive lymphocytes are either deleted or rendered unable to respond by the supposed cell or clone selection mechanism. However, recent findings from a number of laboratories suggest instead of cell selection but receptor selection plays an essential role in immune self-tolerance. Receptor selection is carried out by secondary or nested rearrangement of antigen receptor gene fragments, and can occur at different stages of lymphocyte differentiation. Furthermore, secondary rearrangement of receptor gene also plays an important role in shaping immune response after the interaction of receptor with antigen by altering its specificity.