无名氏:"70 kDa 的蛋白用15% SDS PAGE". I think that may do



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送交者: Wood 于 2006-1-25, 10:52:15:

I mean I am running a 17% SDS PAGE, as the resolving gel, to seperate proteins between 90 kDa ~ 20 kDa. I do this everyday; 17% is not that accurate. So I think 15% might do for that 70 kDa, and it is therefore not as "可悲" as it was in 无名氏's first impression. If 无名氏 later applied the gel to a HPLC-MS system, that is another story.



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