Possible Explanation of Qiu's Inconsistency on Activity



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送交者: Godfather 于 2006-1-20, 12:11:24:

Most likely, in my opinion, the activity was from streptomycin. During the CM-sepharose column, the residual streptomycin was coeluted with his protein (pI~10) since At pH 9, streptomycin is a free base that can bind to the cation exchange resin and be eluted by the similar salt strength. The inconsistency of activity is likely due to the previous dialysis of removing the streptomycin since different length of time and the number of changing dialysis buffer change the residual concentration of streptomycin. I also wonder if streptomycin and colicin Ia can form a complex through hydrophobic interaction or other forces to enhance the antibacterial activity of streptomycin against gram-positive bugs.





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